The wild type and the triple mutant cyca2;234 were grown on control plates or plates containing 1 mM hydroxyurea (HU) or 0.6 μg/ml bleomycin (BLM) for 10 days. Root lengths were measured 10 days after germination. The mutants wee1 and ku70 were used as positive controls for hydroxyurea and bleomycin sensitivity, respectively.
The wild type and cyca2;234 triple mutant germinated on control plates without cisplatin and were transferred to plates containing 30 μM cisplatin 3 days after germination. Roots were measured 3 days after transfer and the net growth of 3 days is shown in the graph.
Images show the wild type and cyca2;234 triple mutant on plates supplemented with 30 μM cisplatin. Images were taken 6 days after germination, that is, 3 days after transfer to cisplatin. Scale bar: 0.5 cm.
Data information: One or two asterisks indicate significant differences within a 5% and 1% confidence interval, respectively (Student's
t‐test). Three biological replicates, each containing at least 15 plants, were analyzed. The mean of the root length of each individual experiment was determined and again averaged for the three biological replicates. Graphs represent mean ± SD.
