Skip to main content
. 2016 Aug 6;35(19):2068–2086. doi: 10.15252/embj.201593083

Figure 7. Mutants of cycb1 ku70 are hypersensitive to BLM: genetic interaction between NHEJ und HR.

Figure 7

  • A, B
    The wild type, ku70, the double mutants cycb1;1 ku70, cycb1;2 ku70, and cycb1;1 cycb1;2, and the triple mutant cycb1;1 cycb1;2 ku70 were grown on control plates (A) or plates containing 0.6 μg/ml BLM (B) and root lengths were measured 10 days after germination.
  • C
    Graph represents the ratio of the mean growth rate on 0.6 μg/ml BLM compared to control experiments on plates lacking BLM for the wild type, ku70, cycb1;1 ku70, cycb1;2 ku70, and cycb1;1 cycb1;2, and the triple mutant cycb1;1 cycb1;2 ku70.
  • D, E
    Image shows the wild type, ku70, the double mutants cycb1;1 ku70, cycb1;2 ku70, and cycb1;1 cycb1;2, and the triple mutant cycb1;1 cycb1;2 ku70 (from left to right) grown on control plates (D) or plates containing 0.6 μg/ml BLM (E) 10 days after germination.
Data information: One asterisk indicates significant differences within a 5% confidence interval between the wild type and the mutants (Student's t‐test). Two asterisks represent significant differences within a 5% confidence interval between the wild type and the triple mutant cycb1;1 cycb1;2 ku70 as well as a significant reduction of root growth between ku70 and the triple mutant cycb1;1 cycb1;2 ku70. Scale bars: 1 cm. Three biological replicates, each containing at least 15 plants, were analyzed. The mean of the root length of each individual experiment was determined and again averaged for the three biological replicates. Graphs represent mean ± SD.