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. 2016 Aug 6;35(19):2068–2086. doi: 10.15252/embj.201593083

Figure EV7. The triple mutant cycb1;1 cdkb1;1 cdkb1;2 shows no additive effect in DNA damage sensitivity.

Figure EV7

  1. The wild type, the cycb1;1 single mutant, the cdkb1;1 cdkb1;2 double mutant, and the cycb1;1 cdkb1;1 cdkb1;2 triple mutant were grown on control plates without genotoxins and plates supplemented with 1 mM hydroxyurea (HU) or 0.6 μg/ml bleomycin (BLM). Root lengths were measured 10 days after germination.
  2. The wild type, the cycb1;1 single mutant, the cdkb1;1 cdkb1;2 double mutant, and the cycb1;1 cdkb1;1 cdkb1;2 triple mutant germinated on control plates and were transferred to plates supplemented with 30 μM cisplatin 3 days after germination. Root lengths were measured 3 days after transfer and the net root growth of 3 days is shown in the graphs.
  3. Images show the wild type, the cycb1;1 single mutant, the cdkb1;1 cdkb1;2 double mutant, and the cycb1;1 cdkb1;1 cdkb1;2 triple mutant (from left to right) 6 days after germination (= 3 days after transfer to 30 μM cisplatin plates).
Data information: One or two asterisks indicate significant differences within a 5 and 1% confidence interval, respectively (Student's t‐test). Scale bars: 1 cm. Three biological replicates, each containing at least 15 plants, were analyzed. The mean of the root length of each individual experiment was determined and again averaged for the three biological replicates. Graphs represent mean ± SD.