Figure EV2. ${\mathbf{\text{PO}}}_{\mathbf{4}}^{-}$ binding sites in Hrr25.

1. Aligned structures of Candida glabrata Hrr25^1–403 crystallized without nucleotide (Apo, left), or in the presence of ADP in crystallization buffer with formate (center) or sulfate (${\text{SO}}_4^{-}$, right). ${\text{PO}}_4^{-}$/${\text{SO}}_4^{-}$ ions bound to sites 1 and 2 are shown as spheres. In the ADP (formate) structure, there is no ion bound to site 2, but a formate ion (CHOO⁻) is observed at site 1.
2. Close‐up view of ${\text{PO}}_4^{-}$ binding sites S1 and S2 in C. glabrata Hrr25 (blue), overlaid with the formate‐bound structure (white).
3. View of the C. glabrata Hrr25 active site and ${\text{SO}}_4^{-}$ at S1, with modeled substrate peptide from a Cdk2:cyclin A:peptide complex (PDB ID 1GY3) (Cook et al, 2002).