. 2016 Aug 5;17(10):1452–1470. doi: 10.15252/embr.201642051

Table 1.

Embryonic development after LNA microinjection

	Experiments (n)	Embryos (n)	2C arrest (%)	4–8C arrest (%)	BL (%)
Control	3	218	7.38 ± 5.17^a	0.56 ± 0.96^j	88.30 ± 7.23^w
Control‐LNA	5	227	10.31 ± 4.59^a	0.32 ± 0.72^j	75.36 ± 2.95^w
LincGET‐LNA1	6	367	86.39 ± 6.72^b	0.00 ± 0.00^j	0.89 ± 1.56^x
LincGET‐LNA2	10	860	90.76 ± 4.28^b	0.00 ± 0.00^j	0.87 ± 1.94^x
LincGET‐LNA3	4	189	76.80 ± 9.72^b	0.00 ± 0.00^j	0.49 ± 0.98^x
Dyei‐LNA	3	149	6.39 ± 2.70^a	0.88 ± 1.52^j	77.67 ± 5.81^w
High6248no	4	255	8.01 ± 1.55^a	7.78 ± 3.17^f	77.80 ± 4.55^w
LincGET‐LNA2 + High2900no	3	186	93.21 ± 1.77^b	0.49 ± 0.84^j	0.00 ± 0.00^x
LincGET‐LNA2 + High2620‐6248	3	106	90.17 ± 3.08^b	1.22 ± 1.28^j	0.00 ± 0.00^x
LincGET‐LNA2 + Low6248no	3	310	57.08 ± 4.17^c	11.78 ± 8.49 ^g	3.41 ± 1.89^y
LincGET‐LNA2 + High6248no	4	421	18.57 ± 1.03^d	50.82 ± 5.76 ^h	5.52 ± 3.07^z
Control‐LNA + si‐Egfp	3	74	4.06 ± 0.26^a	1.45 ± 2.51^j	79.51 ± 5.37^w
Control‐LNA + si‐Hnrnpu	3	84	3.67 ± 0.78^a	2.30 ± 3.98^j	81.21 ± 7.65^w
Control‐LNA + si‐Fubp1	3	83	3.74 ± 0.89^a	0.00 ± 0.00^j	79.69 ± 1.44^w
Control‐LNA + si‐Ilf2	3	79	3.93 ± 0.94^a	2.86 ± 2.58^j	77.37 ± 8.79^w
LincGET‐LNA2 + si‐Egfp	3	106	82.29 ± 4.90^b	0.00 ± 0.00^j	0.00 ± 0.00^x
LincGET‐LNA2 + si‐Hnrnpu	3	118	85.78 ± 5.25^b	0.76 ± 1.31^j	0.00 ± 0.00^x
LincGET‐LNA2 + si‐Fubp1	3	108	54.04 ± 3.51^c	21.24 ± 2.40ⁱ	12.85 ± 1.30^v
LincGET‐LNA2 + si‐Ilf2	3	118	81.44 ± 3.13^b	5.90 ± 1.23^f	0.00 ± 0.00^x
Control‐LNA + OE‐Egfp	3	79	3.04 ± 2.64^a	2.54 ± 2.40^j	81.64 ± 3.77^w
Control‐LNA + OE‐Hnrnpu	3	96	6.16 ± 2.88^e	17.88 ± 7.68ⁱ	62.42 ± 6.41^u
Control‐LNA + OE‐Fubp1	3	84	89.05 ± 1.91^b	0.00 ± 0.00^j	0.00 ± 0.00^x
Control‐LNA + OE‐Ilf2	3	108	8.06 ± 4.90^e	18.31 ± 7.27ⁱ	63.16 ± 6.53^u

2C, two‐cell stage; 4–8C, four‐ to eight‐cell stage; BL, blastocyst stage; 6,248no, full‐length LincGET1 lacking LincGET‐LNA2 target site; 2,900no, 1–2,900 nt of LincGET1 lacking LincGET‐LNA2 target site; 2,620–6,248, 2,620–6,248 nt of LincGET1. High, 400 ng/μl; low, 150 ng/μl. si‐, siRNA. OE‐, overexpression. Different letters in same column indicate significant difference (P < 0.001).