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. 2016 Aug 25;17(10):1396–1409. doi: 10.15252/embr.201541823

Figure EV3. Loss of CITK does not impair the localization of the dynein/dynactin motor complex and NuMA.

Figure EV3

  1. Control and CITK‐depleted cells were immunostained for γ‐tubulin (red), DNA (blue), and NuMA, dynactin (p150), or dynein (green). Images represent maximum‐intensity projections of confocal z‐stacks. Scale bars, 5 μm.
  2. Quantification of fluorescence intensity at spindle poles of NuMA, dynactin (p150), or dynein in HeLa cells transfected with either CTRL or CITK siRNA (n > 150 cells, five independent experiments).
  3. Percentage of mitosis with cortical localization of NuMA or dynactin (p150) in HeLa cells transfected with either CTRL or CITK siRNA. The results shown are representative examples of at least three different experiments.
Data information: Data are presented as mean ± SEM. Statistical significance was assessed using a two‐tailed Student's t‐test. *P < 0.05.