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Guide RNAs used for CRISPR/Cas9
| Guide RNA | Mouse Spata2 gene | Human SPATA2 gene |
|---|---|---|
| gRNA#1 | ATCAGCCGAAATCGATATAA | ATCAGCCGGAATCGATAAAA |
| gRNA#2 | TCAGCCGAAATCGATATAAA | CCGGCTGATCCAGTTCTATG |
| gRNA#3 | CGCAGGTACTCATCGCTGCC | CGCAGGCACTCATCGCTGCC |
| Luciferase | ACCGCTCCGGCGAAGGCGAA | ACCGCTCCGGCGAAGGCGAA |
Three different guide RNAs were used to generate CRISPR/Cas9 vectors, each targeting human or mouse Spata2. As a control, a CRISPR/Cas9 vector targeting luciferase was generated.
