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. 2016 Oct 3;16:230. doi: 10.1186/s12866-016-0855-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — CpG-ODNs induce the expression of TLR9 in SAOS-2 cells. SAOS-2 cells were treated for 4 h with 250 nM of ODN2216 (1), ODN2006 (2), ODN2243 (3) or left untreated (4). cDNA was synthetized from isolated SAOS-2 RNA and underwent real-time quantitative PCR using human TLR9 or ACTB primers. PCR products were analyzed on 1 % agarose gel