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. Author manuscript; available in PMC: 2017 Feb 4.
Published in final edited form as: Nature. 2016 Aug 4;536(7614):81–85. doi: 10.1038/nature18930

Fig. 4.

Fig. 4

In vivo bacterial dynamics, tumor impact, and tolerability in a subcutaneous tumor model. (a) IVIS imaging over time of a mouse bearing two hind flank tumors injected once with the stabilized SLC-hly strain (Strain 8). (b) Single tumor density map trajectories of bacterial luminescence (relative to luminescence at 0h) for the SLC-hly strain (Strain 8). Data for each axis represents separate experiments. (c) Single tumor density map trajectories of bacterial luminescence for the genomically integrated constitutively luminescent strain (Strain 9). Intratumoral injection resulted in over 35-fold higher post-injection luminescence compared to intravenous injection (Extended Data Fig. 3d). (d) Average relative tumor volume over time for subcutaneous tumor bearing mice injected with SLC-hly (red line, Strain 10), SLC-cdd (green line, Strain 14), SLC-ccl21 (blue line, Strain 15), and all together (SLC-3) (black line). Bacteria were injected intratumorally on days 0, 2, 6, 8, and 10 (black arrows) (****P < 0.0001, two-way ANOVA with Bonferroni post test, n = 14 - 17 tumors, s.e.). (e) Average relative tumor volume over time for mice with subcutaneous tumors injected with the SLC-3 strains (black line, Strain 10, 14, and 15) and the no-plasmid control (magenta line, Strain 7). Bacteria were injected intratumorally on days 0, 2, 6, and 10 (black arrows) (****P < 0.0001, two-way ANOVA with Bonferroni post test, n = 18 - 19 tumors, s.e.). (f) Average relative body weight over time for mice with subcutaneous tumors injected with the SLC-3 strains (black line, Strain 10, 14, and 15) and the no-plasmid control (magenta line, Strain 7). Bacteria were injected intratumorally on days 0, 2, 6, and 10 (black arrows) (n=10 mice for both cases, s.e.). (g) Average relative body weight over time for subcutaneous tumor-bearing mice with a single intravenous injection of the SLC + constitutive hlyE (turquoise line, n=9 mice, Strain 11), a non-SLC strain with constitutive hlyE (orange line, n=5 mice, Strain 12), or the no-plasmid control strain (magenta line, n=9 mice, Strain 7) (***P < 0.001, two-way ANOVA with Bonferroni post test, s.e.).

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