Extended Data Fig. 1.

Various properties of the SLC. (a) The fraction and number of bacterial cells cleared per consecutive oscillatory cycle in the growth chamber for a typical microfluidic experiment for S. typhimurium, including the effects of lysis and flow of cells outside of the trap (Strain 1). (b) Subset of time series images from the experiment in (a) showing a portion of the growth chamber where survivors of the initial lysis event (160 min frame, red outline) produce progeny (250 min frame, magenta outline) which are lysis sensitive. (c) Period as a function of the environmental temperature for E. coli (Strain 13). The circuit does not oscillate for temperatures above 37°C in E. coli. Error bars indicate ±1 standard deviation for 12 - 19 peaks. (d) Colony amplitude at quorum firing for increasing degradation on the LuxI activator protein in the computational model. These simulation results are supported by batch well-plate experiments of the LuxI ssrA (black line, Strain 2) and non-ssrA (blue line, Strain 1) tagged versions of the circuit in S. typhimurium (inset).