Table 2. List of down-regulated secreted proteins upon hypovirus infection identified by HPLC-ESI-OrbiTrap MSMS.
| No. | Protein IDa | Protein Name | Average change (EP155/EP713)b | Standard error | Unique PepCountc | Cover Percentd | MWe | pIf |
|---|---|---|---|---|---|---|---|---|
| Hydrolase activity | ||||||||
| 172 | 36288 | Endopolygalacturonase 1 precursor (Pectinase) (Clpg1) | 3.7 (↑) | 0.23 | 4 | 9.46% | 37662.76 | 6.58 |
| 331 | 73874 | Secreted aspartic proteinase | 3.6 (↑) | 0.3 | 2 | 3.55% | 59382.36 | 4.07 |
| 487 | 95002 | Endoglucanase-4 precursor (Endoglucanase IV) (Endo-1,4-beta-glucanase IV) (Cellulase IV) (EGIV) | 3.0 (↓) | 0.32 | 1 | 1.94% | 36058.34 | 4.34 |
| 230 | 108053 | Cutinase precursor (Cutin hydrolase) | 2.8 (−) | 0.42 | 3 | 18.42% | 29225.54 | 4.19 |
| 492 | 69709 | ATP-dependent DNA helicase II, 70 kDa subunit | 2.8 (↓) | 0.09 | 1 | 2.59% | 39932.8 | 4.88 |
| 121 | 105629 | Extracellular chitinase | 2.4 (↑) | 0.13 | 5 | 10.43% | 48705.46 | 4.61 |
| 349 | 43693 | Alpha-glucosidase precursor (Maltase) | 2.4 (↓) | 0.21 | 2 | 1.54% | 111602.21 | 4.49 |
| 480 | 79318 | 3-phytase A precursor (Myo-inositol-hexaphosphate 3-phosphohydrolase A) (3 phytase A) (Myo-inositol hexakisphosphate phosphohydrolase A) | 2.3 (↑) | 0.42 | 1 | 2.08% | 52879.6 | 4.82 |
| 315 | 107379 | Beta-fructofuranosidase | 2.0 (↓) | 0.12 | 2 | 2.74% | 62995.83 | 4.58 |
| 323 | 84745 | Alpha amylase | 2.0 (−) | 0.08 | 2 | 3.17% | 58630.95 | 4.19 |
| 327 | 99792 | Extracellular cell wall glucanase | 2.0 (↑) | 0.36 | 2 | 9.92% | 27691.24 | 4.11 |
| 165 | 67775 | GPI-anchored cell wall beta-1,3-endoglucanase EglC | 1.9 (↓) | 0.07 | 4 | 18.16% | 40242.32 | 4.41 |
| 233 | 107093 | Endothiapepsin precursor (Aspartate protease) | 1.6 (−) | 0.13 | 3 | 6.63% | 56426.06 | 4.43 |
| 305 | 103771 | Survival protein sure-like phosphatase/nucleotidase-like protein | 1.6 (↓) | 0.08 | 2 | 7.79% | 31859.39 | 4.05 |
| 304 | 65827 | Polygalacturonase-3 precursor (Polygalacturonase III) (PG-III) (PGC) (Pectinase-3) | 1.5 (−) | 0.16 | 2 | 10.73% | 36227.1 | 3.8 |
| Oxidoreductase activity | ||||||||
| 474 | 39428 | Short chain dehydrogenase/reductase family oxidoreductase | 2.9 (↑) | 0.2 | 1 | 2.75% | 31682.42 | 6.53 |
| 26 | 106275 | Alcohol dehydrogenase-1(ADH-1) | 2.3 (↑) | 0.1 | 12 | 38.70% | 37278.81 | 6.15 |
| 66 | 39110 | Cellobiose dehydrogenase | 1.8 (−) | 0.08 | 8 | 15.44% | 61477.37 | 5.32 |
| 182 | 97515 | Coproporphyrinogen III oxidase | 1.8 (↓) | 0.17 | 4 | 9.90% | 46496.86 | 7.66 |
| 241 | 71516 | Aspartate-semialdehyde dehydrogenase | 1.7 (−) | 0.2 | 3 | 7.12% | 39149.09 | 6.2 |
| 10 | 101684 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | 1.5 (−) | 0.03 | 18 | 59.76% | 36191.85 | 6.46 |
| 101 | 64525 | NADP-dependent leukotriene B4 12-hydroxydehydrogenase | 1.5 (↑) | 0.1 | 6 | 16.89% | 31463.95 | 6.46 |
| 359 | 83754 | Laccase | 1.5 (↑) | 0.09 | 2 | 3.99% | 63159.75 | 4.36 |
| 388 | 104397 | Putative oxidoreductase | 1.5 (↑) | 0.11 | 2 | 6.67% | 39540.91 | 5.83 |
| Transferase activity | ||||||||
| 345 | 48583 | Serine hydroxymethyltransferase, cytosolic (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT) | 1.8 (−) | 0.22 | 2 | 4.48% | 51419.79 | 6.95 |
| 243 | 108828 | 1,3-beta-glucanosyltransferase gel2 precursor (Glucan elongating glucanosyltransferase 2) | 1.9 (↓) | 0.7 | 3 | 7.79% | 48862 | 4 |
| 325 | 41127 | Ribokinase-like protein | 1.5 (↑) | 0.17 | 2 | 6.80% | 36364.05 | 5.1 |
| Lypase/Esterase activity | ||||||||
| 119 | 105554 | Extracellular lipase | 2.5 (↑) | 0.2 | 5 | 19.08% | 37288.15 | 4.17 |
| 202 | 95432 | Arylsulfatase | 2.3 (−) | 0.07 | 4 | 5.56% | 69618.99 | 4.82 |
| 279 | 97118 | Carboxylesterase | 3.6 (↑) | 0.19 | 3 | 8.23% | 60638.58 | 4.36 |
| Isomerase activity | ||||||||
| 56 | 55987 | Peptidyl-prolyl cis-trans isomerase, mitochondrial precursor (PPIase) (Rotamase) (Cyclophilin) (Cyclosporin A-binding protein) (CPH) | 2.1 (−) | 0.07 | 9 | 43.41% | 20099.5 | 7.81 |
| 244 | 58361 | Peptidyl-prolyl cis-trans isomerase B precursor (PPIase B) (Rotamase B) | 1.7 (−) | 0.29 | 3 | 17.92% | 18877.67 | 6.98 |
| Lyase activity | ||||||||
| 75 | 104720 | fructose bisphosphate aldolase | 1.8 (↓) | 0.24 | 7 | 22.71% | 39353.73 | 5.52 |
| Regulation factors | ||||||||
| 133 | 93073 | mRNA binding post-transcriptional regulator | 1.7 (−) | 0.18 | 5 | 14.66% | 41167.13 | 8.6 |
| 151 | 102966 | 14-3-3 protein | 1.6 (↑) | 0.02 | 5 | 20.51% | 30396.05 | 4.9 |
| 142 | 40411 | RHO protein GDP dissociation inhibitor(RDI) regulation of growth rate | 1.5 (↑) | 0.01 | 5 | 26.87% | 22567.15 | 5.49 |
| Ribosomal proteins | ||||||||
| 261 | 86511 | Ribosomal protein L28e | 2.4 (↑) | 0.26 | 3 | 16.45% | 16509.76 | 11 |
| 55 | 103787 | Ubiquitin-40S ribosomal protein | 1.8 (−) | 0.02 | 9 | 45.81% | 17687.54 | 9.85 |
| Cytoskeleton | ||||||||
| 263 | 76520 | Microtubule-associated protein RP/EB family member 1 | 1.9 (↑) | 0.37 | 3 | 12.70% | 27527.94 | 4.76 |
| 311 | 102150 | Profilin | 1.7 (−) | 0.24 | 2 | 15.91% | 13559.13 | 5.73 |
| Unknown | ||||||||
| 322 | 107212 | uncharacterized protein | 2.9 (↓) | 0.36 | 2 | 9.18% | 29454.98 | 5.74 |
| 313 | 67753 | uncharacterized protein | 2.6 (↓) | 0.16 | 2 | 8.33% | 25705.21 | 4.28 |
| 232 | 74613 | uncharacterized protein | 2.5 (↓) | 0.17 | 3 | 20.33% | 18468.21 | 4.43 |
| 234 | 103448 | uncharacterized protein | 2.3 (↓) | 0.16 | 3 | 13.83% | 40306.61 | 4.8 |
| 100 | 95286 | uncharacterized protein | 1.9 (↓) | 0.14 | 6 | 11.96% | 51317.18 | 9.71 |
| 308 | 74957 | uncharacterized protein | 1.9 (−) | 0.35 | 2 | 9.14% | 36457.53 | 4.08 |
| 301 | 97441 | uncharacterized protein | 1.8 (−) | 0.02 | 2 | 8.14% | 35189.2 | 3.77 |
| 148 | 82357 | uncharacterized protein | 1.7 (↑) | 0.25 | 5 | 17.65% | 23990.76 | 6.65 |
| 312 | 104348 | uncharacterized protein | 1.7 (↓) | 0.2 | 2 | 3.49% | 74787.31 | 4.62 |
| 199 | 107223 | uncharacterized protein | 1.6 (↑) | 0.22 | 4 | 6.58% | 73713.63 | 4.49 |
| 207 | 70844 | uncharacterized protein | 1.5 (−) | 0.23 | 4 | 20.20% | 20542.29 | 4.92 |
aAccession number from Cryphonectria parasitica database v1.0.
bHypovirus-free strain EP155 was set as reference. The average change (EP155/EP713) were from three independent experimental data. ‘(−)’, ‘(↑)’ and ‘(↓)’ mean mRNA expression level of the corresponding gene was unchanged, up-regulated, down-regulated respectively upon hypovirus infection using high-throughput sequencing by Genome Analyzer IIx (Illumina).
cThe total number of unique matched peptides to the identified protein.
dThe cover percentage of matched peptides to the protein sequence.
eTheoretical molecular weight.
fTheoretical pI.