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. 2016 Oct 4;6:34311. doi: 10.1038/srep34311

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Representative images of HIF activity (HRE-Fluc), BRET and fluorescence imaging (FI). BRET and FI images were obtained 1 h after i.v. injection of POL-AF and the HRE-Fluc image was obtained 3 h after BRET imaging. Yellow arrowheads in FI image indicate fluorescence signals from the kidneys. (b) Quantitative analysis of tumor-specific BRET signals from POL-AF. Tumor/Muscle and Tumor/Kidney ratio of BRET and FI signals 1 h after POL-AF injection are shown. n = 6, *p < 0.05. Error bars indicate s.e.m. (c) Correlations between intratumoral HIF-activity (HRE-Fluc bioluminescence) and tumor-specific BRET signals (T/M ratio) 1 h after injection of POL-AF. Each color circle indicates one tumor. n = 17. The results of the same subcutaneous tumors were indicated by the same colored circles. (d) Correlation of spatial intensity distribution between POL-AF BRET and HRE-Fluc or CMV-Fluc bioluminescent images. n = 8. *p < 0.05.