Fig. 7.

Strategy and results of CHART-PCR. a, b Overview on the upstream sequence and core promoters of cel7A/chb1 and cel7B/eg1. The transcription start site is designated as +1. The initiator (Inr) and TATA elements (or box) were illustrated. The three chromatin regions investigated by CHART-PCR are indicated by blue, red, and green bars, respectively. For cel7A/chb1, region 1 covers from +5 to −142; region 2 covers from −143 to −303; region 3 covers from −330 to −470. For cel7B/eg1, region 1 covers from +35 to −138; region 2 covers from −164 to −341; region 3 covers from −369 to −539. The putative DNA-binding sites of CreA and XlnR are indicated by orange and purple triangles, respectively. The orientation of the triangle represents the orientation of the bind motif. Inr, initiator element; TATA, TATAbox. c CHART analysis of cel7A/chb1. d CHART analysis of cel7B/eg1. All values are means from measurements in triplicates and three biological experiments. The error bars indicate standard deviations