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. Author manuscript; available in PMC: 2017 Oct 9.
Published in final edited form as: J Mol Biol. 2016 Jul 29;428(20):3999–4012. doi: 10.1016/j.jmb.2016.07.012

Figure 4.

Figure 4

High resolution views of the cysteine-complexes of Y157F and C93A CDO. 2Fo-Fc electron density and models and interactions are shown as in Figure 2 with occupancies of the bound chloride and Cys provided in parentheses. (A) Cys soak of C93A at pH 8.0 (PDB code 4XF0), the water near Ala93 receives a hydrogen bond from His155 (not shown). (B) Cys soak of Y157F at pH 8.0 (PDB code 4XF1). A Cys plus dithionite soak of Y157F at pH 8.0 yielded virtually identical results (PDB code 4XF3).