Table 2.

Summary of animal body and uterine weights and serum E2 levels

Experiment	Strain	Treatment	Body weight (g) at sacrifice	Uterine weight (mg)	Uterine weight (mg)/Body weight (g)	E2 (pg/μl)
1	C57/BL	Oil fed	23.2 ± 0.5	30.4 ± 1.8	1.3 ± 0.1	12.3 ± 1.5
		E2B fed	24.4 ± 0.3	114.6 ± 8.0 c	4.7 ± 0.4 b	32.8 ± 6.8 a
		Oil fasted	20.4 ± 1.4	39.5 ± 10.9	2.1 ± 0.8	11.7 ± 1.0
		E2B fasted	21.6 ± 0.7	134.9 ± 20.1 d	6.4 ± 1.2 b	86.5 ± 42.2 a
2	C57/BL	LFD oil	28.2 ± 1.4	11.7 ± 1.2	0.4 ± 0.0	10.0 ± 0.4
		LFD E2B	25.3 ± 0.8	108.5 ± 24.6 d	4.3 ± 1.0 d	22.6 ± 1.5 a
		HFD oil	36.6 ± 1.6 B	14.6 ± 2.1	0.4 ± 0.1	11.1 ± 1.1
		HFD E2B	31.7 ± 2.0 a A	119.6 ± 11.3 d	3.9 ± 0.5 d	25.0 ± 5.9 a
3	Swiss Webster	Oil	41.0 ± 2.2	55.7 ± 3.9	1.4 ± 0.2	6.6 ± 0.7
		E2B	44.0 ± 2.5	146.2 ± 11.2 d	3.3 ± 0.2 d	36.4 ± 7.9 c
4	Swiss Webster	Oil fed	36.8 ± 2.7	81.4 ± 3.9	2.2 ± 0.1	3.7 ± 0.5
		E2B fed	40.7 ± 3.2	105.8 ± 22.7 d	2.6 ± 0.9 c	37.8 ± 5.4 d
		Oil fasted	38.0 ± 3.9	76.5 ± 5.9	2.0 ± 0.1	3.5 ± 0.3
		E2B fasted	40.7 ± 4.8	119.7 ± 15.1 d	2.9 ± 0.3 c	24.4 ± 6.2 b A
5	Swiss Webster	LFD oil	37.5 ± 1.5	16.8 ± 1.6	0.5 ± 0.0	2.3 ± 0.3
		LFD E2B	33.3 ± 1.0	177.4 ± 13.6 d	5.4 ± 0.4 d	26.3 ± 6.0 a
		HFD oil	38.4 ± 1.7	23.6 ± 3.8	0.6 ± 0.1	1.8 ± 0.3
		HFD E2B	36.4 ± 1.6	187.3 ± 17.2 d	5.2 ± 0.44 d	18.7 ± 5.6 a
6	Swiss Webster	Oil ad lib	38.2 ± 1.4	24.1 ± 1.6	0.6 ± 0.0	3.9 ± 0.2
		E2B ad lib	40.0 ± 2.1	239.8 ± 16.2 d	6.0 ± 0.3 d	224.8 ± 41.0 d
		Oil CR	33.8 ± 0.8 A	19.2 ± 1.6	0.6 ± 0.0	4.8 ± 0.4
		E2B CR	28.7 ± 1.3 a D	110.2 ± 11.7 d D	3.9 ± 0.5 d D	37.0 ± 19.1 D

Data were analyzed differently in different experiments based on experimental paradigm. See materials and methods for comprehensive statistical method outline. Lowercase letters signify differences between oil- vs. E2B-treated females, within the same treatment (different according to experiment: time of sacrifice, energy balance state, diet). Uppercase letters signify differences within steroid, across treatment. (a/A = p < 0.05; b/B = p < 0.01; c/C = p < 0.001; d/D = p < 0.0001)