Table 3.

Summary table of KNDy-associated gene expression changes in ARC and Tac2 pools.

Gene Name	Fed vs. Fasted* Exp. #1 (ARC) & 4 (pools)				LFD vs. HFD* Exp. #2 (ARC) & 5 (pools)				SW Oil vs. E2B+ Exp. #3	Ad lib vs. CR Exp. #6
	Fed vs. Fasted		Oil vs. E2B		LFD vs. HFD		Oil vs. E2B			Ad lib vs. CR		Oil vs. E2B
	Oil	E2B	Fed	Fasted	Oil	E2B	LFD	HFD		Oil	E2B	Ad lib	CR
Kiss1	ARC		ARC	ARC	ARC		ARC		ARC	ARC		ARC	ARC
Tac2*			pools	□			ARC	ARC pools	ARC	ARC		ARC
Pdyn										ARC	ARC	ARC	ARC
Kiss1r		ARC				ARC				ARC			ARC
Tac3r*			ARC	ARC		ARC
Kor	ARC		ARC
Esr1*
Pgr*			ARC pools	ARC pools			pools	pools	ARC			ARC	ARC
Fig.	ARC: Fig. 2; pools: Fig. 4D				ARC: Fig. 3; pools: Fig. 4F				Fig. 4A	Fig. 5

Experiments and genes with a * indicate qPCR analysis of ARC and Tac2 pools. Experiments without * were only analyzed in ARC tissue. For all experiments except oil vs E2B (₊), data were analyzed with using a two-way ANOVA followed by post-hoc Newman-Keuls. For oil vs. E2B experiment, data were analyzed with a Student’s t-test Corresponding graphs of each gene expression change is noted in the “Fig.” row. Arrows indicate the direction of gene expression ( = upregulated and = downregulated compared to control (oil, fed, LFD, ad lib). For example, in experiment #1 (fed vs. fasted) Kiss1 in the ARC is increased by fasting in oil-treated animals (Fig. 2A) and decreased by E2B in both fed ans fasted animals (Fig. 2A).