Overlapping but distinct distribution of α-Gustducin (green) and Vimentin (red) immunosignals in taste buds in the base of the oral cavity at P1 (A), P3 (B), and P5 (C, D). White dots outline the specified taste bud cell clusters. Arrows in A–C point to the connective tissue. Arrows in D point to the unlabeled cells. Arrowheads point to cells double labeled with both α-Gustducin and Vimentin. Open arrowheads in A–B point to singly labeled α-Gustducin+ or Vimentin+ cells. Asterisks mark non-specifically labeled cells. Scale bars: 20 µm for all images (A–C), X=Y=10 µm, Z= 8 µm (D) (single plane laser-scanning confocal in A–C, 3D confocal in D).