Table 1.
Primers (Position)a | Sequence |
---|---|
17-mer (−4) | 5′-AACGACGGCCAGTGAAT-3′ |
18-mer (−3) | 5′-AACGACGGCCAGTGAATT-3′ |
19-mer (−2) | 5′-AACGACGGCCAGTGAATTC-3′ |
20-mer (−1) | 5′-AACGACGGCCAGTGAATTCG-3′ |
21-mer (0) | 5′-AACGACGGCCAGTGAATTCGC-3′ |
22-mer (+1) | 5′-AACGACGGCCAGTGAATTCGCG-3′ |
Templates | |
26-mer | 3′-TTGCTGCCGGTCACTTAAGCGCGCCC-5′ |
26-mer-dGC8-N-ABA | 3′-TTGCTGCCGGTCACTTAAGCGCGCCC-5′ |
G indicates the dGC8-N-ABA lesion at the 21st position in the template.
Position of primer 3′ terminus relative to the templating dGC8-N-ABA.