. Author manuscript; available in PMC: 2017 Oct 1.

Published in final edited form as: DNA Repair (Amst). 2016 Sep 1;46:20–28. doi: 10.1016/j.dnarep.2016.08.002

Table 5.

Kinetic parameters from biphasic kinetic assays with hPolη at 37 °C

[dNTP]	A_f (nM)	k_f (s⁻¹)	A_s (nM)	k_s (s⁻¹)
20/26-mer-dG^C8-^N^-ABA
300 μM dCTP	5.6 ± 0.8 (19%)	4.6 ± 1.3	8.3 ± 0.8 (28%)	0.32 ± 0.06
600 μM dCTP	5.5 ± 0.7 (18%)	6.3 ± 1.5	8.5 ± 0.7 (28%)	0.36 ± 0.05
1000 μM dCTP	5.8 ± 0.8 (19%)	7.2 ± 1.4	8.6 ± 0.8 (29%)	0.42 ± 0.06
21/26-mer-dG^C8-^N^-ABA
25 μM dGTP	4.4 ± 0.7 (15%)	3.8 ± 1.5	9.2 ± 0.8 (31%)	0.082 ± 0.002
50 μM dGTP	4.4 ± 0.8 (15%)	5.2 ± 1.7	9.1 ± 0.6 (30%)	0.13 ± 0.02
90 μM dGTP	4.3 ± 0.6 (14%)	11 ± 4	9.3 ± 0.7 (31%)	0.16 ± 0.03

A_f and A_s are the reaction amplitudes of the fast and slow phases, respectively, while k_f and k_s are the rate constants of the fast and slow phases, respectively.