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Fig. 4. Western Blot analyses of key proteins of the sHPA axis.
CRF, CRFR1 and MC2R protein levels were measured in primary human epidermal keratinocytes stimulated with 1,25(OH)2D3 (0.1 µM), Ca2+ (2.5 mM), or both, for 24 h. β-Actin levels were measured as a control.