Figure 7.

Inhibition of capsaicin induced Ca²⁺ influx in TRPV1-HEK293 cells by myristicin. TRPV1-HEK293 cells were treated with 100 nM capsaicin (Cap) to establish the basal transient Ca²⁺ influx response. The cells were then treated with 1 µM (Panel A), 10 µM (Panel B), and 100 µM (Panel C) myristicin followed by 100 nM capsaicin (Cap) to evaluate response desensitization, as described. Panel D. To confirm that the initial application of capsaicin did not desensitize TRPV1, cells were treated with 100 nM capsaicin (Cap) followed by a second treatment of 100 nM capsaicin (Cap) at the same interval that was used in panels A–C. Data from three representative experiments are shown.