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. 2016 Oct 4;2:16033. doi: 10.1038/celldisc.2016.33

Figure 1.

Figure 1

ASK1 localizes to spindle poles and is important for proper cell division. (a) Immunofluorescence images of HeLa cells stained with anti-ASK1 and anti-α-tubulin antibodies and DAPI. Scale bars, 5 μm. (b) Immunoblots for ASK1 and α-tubulin expression in control and ASK1 siRNA-treated HeLa-H2B cells. (c) Time-lapse images showing prolonged metaphase and telophase/cytokinesis and uneven timing of daughter cell adhesion to the substratum in ASK1 siRNA-treated HeLa-H2B cells. Dashed lines indicate daughter cells that are not in the same focal plane. The unit for time is minute. (d) Duration of different mitotic phases in cells treated as in (c). n=10 cells per group. (e) Quantification of cell divisions with uneven timing of daughter cell adhesion to the substratum in cells treated as in (c). n=10 cells per group. Scale bars, 5 μm. Experiments were performed three times. Values are mean±s.e.m. **P<0.01, ***P<0.001; NS, not significant.