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SIRPα is upregulated in tumor-associated DCs. (A & B) Mouse BMDCs from C57BL/6 mice were incubated with 100 ng/mL LPS, 5 μg/mL CpG or 0.25 μg/mL polyI:C for the indicated time, and SIRPα protein expression levels were ascertained by Western blotting. (C) FACS analysis of surface expression of SIRPα in CD11c+ DCs infiltrated in HCC and in autologous CD11c+ PBDCs. PBDCs from five normal healthy people were used as control.
