Figure 2.

Adoptive transfer of CEA-specific CAR-T cells eradicated established tumors in an antigen-specific manner. (A) Schematic representation of the adoptive transfer experiment using CEA-specific CAR-T cells. (B) Tumor growth curves of mice transferred with CAR-T cells. WT and CEA-Tg mice bearing 7-d-old MC38 or MC32a tumors were transferred with CEA-specific CAR-T cells. Some mice also received lymphodepleting preconditioning as indicated. Tumor volumes were measured by a calliper using the formula (length × width) at the indicated time points (n = 5). *p < 0.05, **p < 0.01. (C) In vivo persistency of transferred CAR-T cell in tumor-bearing CEA-Tg mice. Peripheral blood of CEA-Tg mice as in (A) was collected at day 17 after tumor inoculation by retro-orbital bleeding, pooled (n = 5), and subjected to flow cytometry analysis.