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. 2016 Jul 25;5(9):e1211218. doi: 10.1080/2162402X.2016.1211218

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — Weight loss seen in preconditioned CEA-Tg mice transferred with CEA-specific CAR-T cells was caused by anorexia. (A) Increased serum levels of cytokines associated with anorexia. Pooled serum samples from mice (n = 5) were collected at day 17 after tumor inoculation and subjected to analysis by cytokine beads arrays. (B) Reduced diet consumption of preconditioned CEA-Tg mice transferred with CEA-specific CAR-T cells. Diet consumption of CEA-Tg and WT mice were measured at the indicated time points and the measured quantity was divided by the number of mice to determine the quantity of consumed food by each mouse. (C) CEA-Tg mice, but not WT mice, suffered from malnutrition and liver function of CEA-Tg and WT mice. Pooled serum samples (n = 5) were subjected to serum chemistry analysis.