Figure 3.

CpG ODN can enhance cytokine secretion by HJ1 T cells in vitro. WT (open bars) and hCD1Tg BMDC (black bars) were either left untreated or pretreated with TLR agonists- Pam₃Cys, poly (I:C), R848 and CpG ODN prior to co-culture with enriched hepatic HJ1 T cells from hCD1Tg/HJ1Tg/Rag2^−/− mice. ELISA was performed to detect IFNγ (A) and IL-13 (B) secretion in the supernatant 24 h later. Data are representative of two independent experiments. (C) The expression of IL-12R, IL-18R, and TLR 9 on purified iNKT cells (open bars) and HJ1 T cells (black bars) was determined by detecting mRNA levels relative to β-actin using quantitative RT-PCR. (D) Hepatic HJ1 T cells were co-cultured with CpG ODN-treated hCD1Tg BMDC for 48 h in the presence of anti-IL-12 and/or anti-IL-18 or in the presence of CTLA-4 Ig. IFNγ ELISA was performed upon collection of supernatant. Data shown are representative of two independent experiments and error bars represent the mean ± SEM. ***p < 0.005; **p < 0.01; *p < 0.05.