Skip to main content
. 2016 Jul 15;5(9):e1211220. doi: 10.1080/2162402X.2016.1211220

Figure 3.

Figure 3.

Simultaneous and specific antigen binding of a triplebody ULBP2-aCD19-aCD33. Simultaneous binding of ULBP2-aCD19-aCD33 to all three target moieties CD19, CD33 and NKG2D was detected. CD19+ MEC1 cell line was incubated with ULBP2-aCD19-aCD33 and its CD19 specific binding to MEC1 cells was detected using recombinant human NKG2D-Fc and CD33-FLAG receptors followed by AF647 labeled anti-Fc and PE labeled anti-FLAG antibodies, respectively (U-19-33). Binding of ULBP2-aCD19 to CD19 on MEC1 cells was detected only using NKG2D-Fc as it lacked anti-CD33scFv (U-19). ULBP2-aCD33 was used as a negative control that failed to bind CD19+ MEC1 cells (U-33) whereas NKG2D-Fc and CD33-FLAG background binding was minimal (No IL).