Table 1.
Biometric parameters of germfree, SIHUMI, and SIHUMI‐Bif mice fed a low fat diet (LFD), a high fat diet (HFD), or a high fat diet with oligofructose (HFD + OFS) for five weeks
| Status | Germfree | SIHUMI | SIHUMI‐Bif | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Diet | LFD | HFD | HFD + OFS | LFD | HFD | HFD + OFS | LFD | HFD | HFD + OFS |
| Biometric parametera | Mean ± SEM | Mean ± SEM | Mean ± SEM | Mean ± SEM | Mean ± SEM | Mean ± SEM | Mean ± SEM | Mean ± SEM | Mean ± SEM |
| Body fat (week 5; g) | 8.5±0.3 | 10.1±0.5* | 7.5±0.6++ | 7.6±0.3 | 10.5±0.9** | 7.9±0.3+ | 9.5±0.5‡‡ | 11.9±0.6* | 9.4±0.5+ |
| Lean mass (week 5; g) | 17.4±0.6 | 17.8±0.5 | 14.4±1.1*+ | 19.6±0.2† | 19.5±0.4† | 19.0±0.3†† | 20.3±0.6†† | 20.9±0.4††† | 21.1±0.9††† |
| Energy intake (week 2; kJ/d) | 55.1±1.8 | 60.1±3.5 | 47.0±3.0+ | 51.9±8.0 | 63.9±6.4 | 53.0±5.6 | 58.7±6.0 | 64.1±8.6 | 73.0±6.9††‡ |
| Digestibility (%) | 77.4±0.8 | 85.9±0.8*** | 82.8±1.2** | 81.4±3.4 | 90.1±1.2† | 75.0±4.9+ | 78.9±1.5 | 88.4±0.5 | 75.5±5.2+ |
| Digestible energy (week 2; kJ/d) | 43.3±1.6 | 51.7±3.4 | 39.9±2.7+ | 47.4±3.8 | 57.8±6.3 | 41.2±6.5 | 46.5±5.2 | 63.7±3.6 | 56.4±6.7 |
| Liver triglycerides (mg/g tissue) | 7.4±1.5 | 10.9±0.7 | 7.7±2.9 | 6.5±0.9 | 12.1±1.2** | 7.4 ±1.2+ | 10.0±1.5 | 12.2±1.5 | 7.5±1.1 |
a
Data are presented as means with their standard errors (n = 6–7 per dietary group, except for body fat of germfree mice fed HFD + OFS n = 3). SIHUMI = simplified human microbiota, SIHUMI‐Bif = SIHUMI without Bifidobacterium longum. Mean values were significantly different compared with LFD (*) and HFD (+) fed mice with the same bacterial status (*+ p < 0.05, **++ p < 0.01, ***+++ p < 0.001). Mean values were significantly different compared with germfree (†) and SIHUMI (‡) mice on the same diet (†‡ p < 0.05, ††‡‡ p < 0.01, †††‡‡‡ p < 0.001).