Table 1.
Sumoylation Targets of the Cytoskeleton
| Polymer system | Type of modification | Site of modifcation | Evidence | Reference |
|---|---|---|---|---|
| Septins | ||||
| Cdc3p | Covalent | K4, K11, K30, and K63 | Pulldown | Johnson and Blobel [1999] |
| Cdc11p | Covalent | K412 | Pulldown | Johnson and Blobel [1999] |
| Shslp | Covalent | K426, K437 | Pulldown | Johnson and Blobel [1999] |
| Microtubules | ||||
| Alpha‐beta tubulin | Covalent | N/A | Proteomic screens, pulldown | Panse et al. [2004]; Wohlschlegel et al. [2004]; Rosas‐Acosta et al. [2005] |
| CENP‐E | Covalent and non‐covalent | N/A | Pulldown | Zhang et al. [2008] |
| Ndc80 | Covalent | K231 | Proteomic screens, pulldown | Panse et al. [2004]; Zhou et al. [2004]; Wykoff and O'Shea [2005]; Montpetit et al. [2006] |
| Tau | Covalent | K340 | Pulldown | Dorval and Fraser, [2012, 2006]; Takahashi et al. [2008] |
| Pac1p | Covalent | N/A | Pulldown, Y2H | Alonso et al. [2012] |
| Kar9p | Covalent | K301, K333, K381, and K529 | Pulldown, Y2H | Leisner et al. [2008]; Meednu et al. [2008] |
| Bik1p | Covalent | N/A | In vitro, Y2H | Alonso et al. [2012] |
| Bim1p | N/A | N/A | Y2H | Meednu et al. [2008] |
| La | Covalent | K41 | Pulldown | van Niekerk et al. [2007] |
| Microfilaments | ||||
| Actin | Covalent | K68, and K284 | Proteomic screens, pulldown | Panse et al. [2004]; Vertegaal et al. [2004]; Wohlschlegel et al. [2004]; Rosas‐Acosta et al. [2005]; Hofmann et al. [2009] |
| Rac1 | Covalent | K188, K183, K184, and K186 | Pulldown | Castillo‐Lluva et al. [2010] |
| RhoGDI | Covalent | K138 | Pulldown | Liu et al. [2011]; Yu et al. [2012] |
| Arc35p | Covalent | N/A | Proteomic screen, pulldown | Wohlschlegel et al. [2004]; Sung et al. [2013] |
| Arc40p | Covalent | N/A | Proteomic screen | Wohlschlegel et al. [2004] |
| Arc19p | Covalent | N/A | Proteomic screen | Nie et al. [2012] |
| Arc15p | Covalent | N/A | Proteomic screen | Nie et al. [2012] |
| Intermediate filaments | ||||
| Vimentin | Covalent | N/A | In vitro, pulldown | Wang et al. [2010], Snider et al. [2011] |
| Keratin 8 | Covalent | K285, K364 | In vitro, pulldown | Snider et al. [2011] |
| Keratin 18 | Covalent | K207, K373 | In vitro, pulldown | Snider et al. [2011] |
| Keratin 19 | Covalent | K208 | In vitro, pulldown | Snider et al. [2011] |
| Lamin A | Covalent | K201, K420, and K486 | Pulldown, Y2H | Zhang et al. [2008], Galisson et al. [2011], Simon et al. [2013] |