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Application of ps-pH-neutral in bacterial culture. Bacterial culture containing pH sensors were incubated in a sealed cuvette at 37 °C. The fluorescence spectra of pH sensors were dynamically detected by spectrofluorophotometer under 455 nm excitation, A: L. fermentum, C: E. coli, E: B. subtilis. The pH values (red) were transferred from their corresponding spectra data and were plotted together with their growth curve (black), B: L. fermentum, D: E. coli, F: B. subtilis.
