Application of ps-pH-neutral in the high throughput detection of bacterial culture with microplates. Bacterial culture containing pH sensors were incubated in microplates at 37 °C. The fluorescence intensities at 475 nm and 505 nm of pH sensors were dynamically detected by a microplate reader under 455 nm excitation, A: L. fermentum, C: E. coli, E: B. subtilis. The ratio values (I475nm/I505nm) were calculated and were plotted vs. incubation time, B: L. fermentum, D: E. coli, F: B. subtilis.