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. 2016 Sep 26;26(18):2486–2491. doi: 10.1016/j.cub.2016.07.013

Figure 2.

Figure 2

Impact of Natural OH Cell Activity on Spontaneous Feeding Rhythm

(A) Strategy for destroying OH neurons in adult mice.

(B) Immunostaining for OH (green) and melanin-concentrating hormone-containing (MCH) (red) neurons in DTR− (top) and DTR+ (bottom) littermates 10 days after DT injection. The DT injection led to the loss of OH neurons in all brains tested (n = 8). Scale bars represent 500 μm (left) and 100 μm (right). Dashed boxes in the left-hand panels indicate the areas shown in corresponding right-hand panels.

(C) Time course of OH cell loss after DT injection in DTR+ and their DTR− littermates (n = 5 mice in each group).

(D) Quantification of OH cell number >21 days after DT injection in DTR− and DTR+ littermates. Unpaired t test, t(13.3) = 16.41, p = 3.2e-10, n = 8 mice in each group.

(E) Body weight time series of DTR− and DTR+ littermates after DT injection. ANCOVA, F(1, 12) = 12.07, p = 0.005, n = 7 mice in each group.

(F) Daily rhythm of eating in DTR− and DTR+ mice after DT injection, across days. n = 7 mice in each group.

(G) Mean daily rhythm of eating (average of 14 days; gray box is lights off) in DT-injected DTR− and DTR+ mice. Repeated-measures ANOVA, interaction: F(7, 84) = 2.38, p = 0.029. Significant differences were found only at the time of day indicated (∗∗∗p < 0.001, Holm correction for multiple comparisons). n = 7 mice in each group.

(H) Total food consumed after DT injection, relative to the time of day. Repeated-measures ANOVA, interaction: F(7, 84) = 3.07, p = 0.006. Pairwise comparisons revealed statistical differences at the time of day indicated (∗∗∗p < 0.001, Holm correction for multiple comparisons). n = 7 animals in each group.

See also Figure S4.