Figure 2.

dsx⁺ Neurons in the Female Brain Evoke Pulse and Sine Song

Courtship song was detected in recordings of 10 min. Sine song is shown in blue and pulses in red.

(A) Close up of courtship song trace produced by a wild-type male courting a wild-type virgin female at 33°C.

(B and C) Close up of courtship song traces produced by a dsx^brain>TrpA1 female paired with a wild-type male (B) or female (C) at 33°C. Timescale indicated in milliseconds (ms).

(D–G) Boxplots of individual flies’ pulse song frequency in Hz (D), number of cycles per pulse (E), sine song frequency in Hz (F), and mean IPI in ms (G).

Median with interquartile range is indicated by boxes. Mean values are indicated below each box. n = 20 flies recorded. Kruskal-Wallis ANOVA was performed for all assays (^∗∗∗p < 0.0001). IPI, inter-pulse interval. See also Figure S2.