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. 2015 Aug 21;64(1):90–104. doi: 10.1002/glia.22905

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© 2015 The Authors. Glia Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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The inner subventricular zone (ISVZ) of occipital cortex from a 21st wpc human fetus (CRL: 200 mm). Double immunolabeling with antibodies against SSEA‐4 (red) and YKL‐40 (green), with nuclei labeled with DAPI (blue). (A) The SSEA‐4 and YKL‐40 positive cells are oriented in the same direction on a migratory trajectory. Merged images of double‐immunolabeled cells are boxed in (A) and shown at higher magnification in (D) and (G), with individual channels in (B), (C) and (E), (F), respectively. The double‐labeled cells show immunoreactivity within the same cell compartments (ER/Golgi apparatus) in close apposition to the nucleus [(D), (G)]. Abbreviations: ISVZ: inner subventricular zone. Scale bar: 10 µm. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]