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. 2015 Aug 24;58(1):29–47. doi: 10.1111/jipb.12371

Figure 7.

Figure 7

Effects of 10 mM H2O2 on detached leaves from wild‐type (wt) and GR2 RNAi (igr2‐7, igr2‐9, and igr2‐14) plants (A) Morphologies of leaves. Bars = 5 mm. (B) Chlorophyll content, Fv/Fm, MDA content, and membrane ion leakage. (C) Expression of the senescence‐related marker genes, SAG12 and SAG13. Transcript levels were determined by real‐time PCR using ACT2 as an internal control. For SAG13, the relative transcript levels detected in wt after H2O2 treatments were assigned a value of 1 after normalization to the ACT2 transcript levels. For SAG12, the relative transcript levels detected in wt after H2O2 treatments were assigned a value of 1 after normalization to the ACT2 transcript levels. SAG12 and SAG13 expression was not detected in wt and RNAi plants in the absence of the H2O2 treatment. The fourth rosette leaves at 20 d after emergence were used in the experiments. The values are means ± SD of three independent experiments.