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. Author manuscript; available in PMC: 2016 Nov 16.
Published in final edited form as: Nat Cell Biol. 2016 May 4;18(6):700–708. doi: 10.1038/ncb3347

Figure 6. Self-organisation of hiPSCs in response to extracellular matrix signaling.

Figure 6

hiPSCs were plated in a 3D matrix of matrigel and analysed at the indicated time points. a-b, hiPSCs stained for aPKC, OCT-4 and F-actin. c, hiPSCs stained for PAR-6, GM130 and F-actin. d, hiPSCs cultured in the presence of the ROCK inhibitor Y-27632 and stained for aPKC and F-actin. e-f, hiPSCs cultured in the presence (e) or absence (f) of ROCK inhibitor, and stained for PODXL and F-actin g, hiPSCs were treated with the caspase 3 inhibitor Z-DEVD FMK and lumen formation was analysed 24 and 48 hours after plating by staining for aPKC and F-actin. h, Quantification of lumen formation in the presence of Z-DEVD FMK. Data is shown as a contingency table. Fisher’s exact test. ns: not significant (n=12 hiPSC organoids per condition -24h time point-; n=10 hiPSC organoids per condition -48h time point-). Data presented in this figure involved the assessment of a minimum of 10 hESC organoids per panel and per condition across a minimum of 2 independent experiments per panel.