Skip to main content
. 2015 Sep 14;12(2):375–382. doi: 10.1080/21645515.2015.1090069

Table 2.

Target genes and primer and probe sequences used for qPCR assays. BHQ: Black Hole Quencher. *:Primer design from this study

Organism Gene Forward Primer Sequence 5′ - 3′ Reverse Primer Sequence 5′ - 3′ [Dye]ProbeSequence 5′ - 3′
S. pneumoniae31 lytA ACGCAATCTAGCAGATGAAGCA TCGTGCGTTTTAATTCCAGCT [Cy5]GCCGAAAACGCTTGATACAGGGAG[BHQ2]
S. pyogenes* ntpC TGCACCAGTCAGTCTATCACC AGCGATAGGTCTTGGTTAGCTC [6FAM]TGACGATCTCAACGATTTGGATCGG[BHQ1]
M. catarrhalis36 ompJ CAGCCTAGCAGGCGGTGTT TTGCTTCAACGCCCACATT [VIC]TTGGCTTTAAACCATTAGC[MGB]
H. influenzae* hdp TTGGCCCAGGTTGGTATATG TTACGCACGGTGTAAGGATG [6FAM]CACTCCGTTGGTAAAAGAACTTGCACA[BHQ1]
S. aureus33 nuc GTTGCTTAGTGTTAACTTTAGTTGTA AATGTCGCAGGTTCTTTATGTAATTT [6FAM]AAGTCTAAGTAGCTCAGCAAATGCA[BHQ1]