Figure 5.

ISM expression by cellular vaccines significantly reduced average tumor burden and prolonged tumor-free survival. (A) Mice were challenged (s.c.) with 2 × 10⁵ live D2F2/E2 WT cells. One week later, mice received one dose of 2 × 10⁵ irradiated cellular vaccines on the contralateral flank (s.c.). (B) Tumor growth and tumor free survival was assessed. (C) D2F2/E2 cells cultured in vitro (Cultured Cells, solid black line) and freshly isolated tumors harvested from WT challenged mice (Tumor, dashed line) were assessed for the expression of PD-L1 by flow cytometry analysis (MFI-mean fluorescence intensity). (D) Splenocytes and tumor infiltrating lymphocytes (TILs) from tumor-bearing mice were isolated and analyzed for PD1 expression on CD8⁺ and CD4⁺ T cells by flow cytometry, mean fluorescence intensity (MFI). Data is representative of 2 individual experiments. Significance relative to PBS-treated. *p < 0.05, **p < 0.01, ***p ≤ 0.001.