Figure 2.

Antibody response at 35 and 100 dpi in mice receiving the immunotherapeutic vaccine during the acute phase of T. cruzi infection. Mice were challenged with RA trypomastigotes and were treated by intramuscular route with 2 doses of nEmpty, nGM-CSF,nCz or nCz+pGM−CSF. Serum samples were taken on day 35 and 100 postinfection. (A) IgG titers against rCz or parasite antigen fraction F105.(B) Immunoblotting of epimastigotes blotted onto nitrocellulose incubated with dilution of mice sera at (I) 35 dpi and (II) 100 dpi; (1) Molecular weight marker, (2 and 3) Control, (4 and 5) Cz+GM−CSF. Strips were incubated with biotinylated rat monoclonal antibodies to mouse IgG1 (2 and 4) or IgG2a (3 and 5) subclasses. (C) Cz specific IgG1 and IgG2a isotypes. The bars represent the media of 7 determinations and are representative of 3 independent experiments.*p < 0.05; **p < 0.01; ***p < 0.001.