Figure 4.
Protection against T. cruzi, in mice treated during the acute phase of infection with Cz and GM-CSF DNAs delivered by attenuated Salmonella. C3H/HeN mice infected with a lethal dose of RA trypomastigotes were treated at 0 and 10 dpi with 2 oral doses of 109 UFC of SCz, SGM-CSF or both combined SCz+SGM−CSF. As control, a group of mice was treated with Salmonella transfected with a plasmid without insert (SEmpty). (A) Parasitemia determined every 2–3 d in fresh blood. The symbols represent the media ± the standard error. (B) Survival recorded daily. These results were representative of 3 independent experiments. *p < 0.05.