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. 2016 Oct 4;11(10):e0163967. doi: 10.1371/journal.pone.0163967

Fig 1. PCDH1 localizes to cell-cell contact sites in 16HBE cells.

Fig 1

(A-C) To examine the subcellular localization of the PCDH1 isoforms (arrows), 16HBE cells were transfected with either green fluorescent protein GFP-tagged PCDH1-isoform-1 (A), PCDH1-isoform-2 (B) or control GFP-tagged cells (C). Representative images are shown from confocal z-stacks showing orthogonal cross-sections at the lines marked, and overlaid with DAPI nuclear stain. Green = PCDH1 isoform-1 or isoform-2; Blue is DAPI (nucleus). Scale bars, 30 μm. (D-E) Representative immunofluorescence overlaid images showing 16HBE cells untreated (D) and transfected with PCDH1-siRNA (E), both stained with PCDH1-EC1 antibody followed by detection with secondary goat anti-mouse Alexa Fluor 633 conjugate antibody (red) and nuclear staining with DAPI (blue). Scale bars, 50 μm.