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. 2016 Oct 4;11(10):e0163922. doi: 10.1371/journal.pone.0163922

Fig 2. Effect of Cl-amidine treatment on immune cell accumulation and activation in adipose tissue and liver.

Fig 2

A) White adipose tissue was digested by 0.25 mg/mL Liberase to isolate the resident immune cells. Flow cytometry analysis was performed for leukocytes (CD45+), myeloid cells (CD45+/CD11b+), neutrophils (CD45+/CD11b+/Ly6G+), macrophages (CD45+/CD11b+/F4/80+) and expression of macrophage pro-inflammatory markers CD11c and MHCII on macrophages. B) Similar flow cytometry was performed on liver resident immune cells. Data is represented as mean ± SEM, n = 10 for each group unless stated otherwise.