Fig. 1.
Identification and verification of miRNAs differentially expressed in the HRECs isolated from control and diabetic donors
(A) Human RT2 miRNA PCR arrays were used to profile the differential expression of total miRNAs in HRECs isolated from control (n = 6) and diabetic donors (n = 6). MiRNAs upregulated or downregulated by > 2.5-fold relative to control are shown. MiRNAs marked with red or green were top 15 miRNAs upregulated or downregulated by diabetes, respectively. (B) Relative expressions of miR-15a and selected miRNAs were determined by qPCR to validate the array result. Data are mean ± SEM (n = 6). *** P < 0.001; ** P < 0.01; * P < 0.05; not significant at P > 0.05.