Fig. 3.

Stored human and murine HbAS RBCs show accelerated post-transfusion clearance compared to stored HbAA RBCs. (a) WT C57BL/6 recipient mice (n = 6) were transfused with a 50:50 mixture of fresh DiI-labeled h-HbAA RBCs (indicated as red triangle) and fresh DiD-labeled h-HbAS RBCs (indicated as black circle). (b) N = 9 WT C57BL/6 recipients were transfused with a 50:50 mixture of 39-day stored DiI-labeled h-HbAA RBCs (indicated as blue circle) and stored DiD-labeled h-HbAS RBCs (indicated as green square) both re-suspended to a 55% hematocrit following labeling. (c) WT C57BL/6 mice recipients (n = 6) were transfused with a 50:50 mixture of fresh DiI-labeled m-HbAA RBCs (indicated as red triangle) and fresh DiD-labeled m-HbAS RBCs (indicated as black circle). (d) WT recipient mice (n = 6) were transfused with 11-day stored DiI-labeled m-HbAA RBCs (indicated as blue circle) and DiD-labeled stored m-HbAS RBCs (indicated as green square). All mice were 8–12 weeks of age and received a total volume of 200 μl of leukoreduced HbAA and HbAS RBCs (100 μl each). To mimic similar conditions, human and murine RBCs were stored in glass. Post-transfusion recovery was measured by dual-label cell tracking by flow cytometry unless stated otherwise. The results are presented as mean ± SD. *p < 0.05; ***p < 0.001; ****p < 0.0001 analyzed by 2Way ANOVA, GraphPad Prism 6.0.