Fig. 4.

Clodronate treatment or splenectomy does not alter stored m-HbAS RBC post-transfusion survival. (a) WT recipient mice were treated with clodronate or PBS liposomes 24 h prior to liver and spleen harvest for immunohistochemistry. Representative images are shown indicating staining for F4/80 + macrophages. (b) WT mice received either clodronate, PBS liposomes or no treatment. Mice were euthanized after 24 h. Spleens were homogenized and stained with F4/80 and CD11b antibodies. Samples were analyzed using flow cytometry to quantify the F4/80 + population. (c) WT recipient mice were transfused with 11-day stored m-HbAS RBCs 24 h following clodronate (n = 3) or PBS liposome (n = 3) injection (2 mg i.p.). (d) WT recipient mice were transfused with 11-day stored m-HbAS RBCs 5 d following splenectomy (n = 4) or sham procedure (n = 6) Results presented are mean ± SD.