Fig. 5.

Splenectomy does not increase kidney and liver sequestration of stored m-HbAS RBCs following transfusion. Splenectomized WT C57BL/6 recipient mice (n = 7) or sham operated (n = 5) were transfused with stored m-HbAS RBCs. Kidney and liver organs were harvested 2 h post-transfusion, fixed in 2% PFA and processed for confocal imaging. (a) Confocal images showing sequestered m-HbAS RBCs in recipient mouse tissues (Magnification 20 ×, Green: phalloidin, Blue: DAPI, Red: Cy3-labeled m-HbAS RBCs). (b) Individual points represent sequestered m-HbAS RBCs normalized to nuclei number per section quantified by fluorescence intensity using NIS Elements. Two sections from each recipient mouse organ were analyzed.