Fig. 6.

Increased tissue sequestration of stored m-HbAS RBCs within kidney, liver and spleen organs following transfusion when compared with stored m-HbAA RBCs. WT C57BL/6 recipient mice (n = 5 per group) were transfused with stored cy3 labeled m-HbAA or m-HbAS RBCs. Kidney, liver and spleen organs were harvested 2 h post-transfusion for confocal imaging. (a) Confocal images showing sequestered m-HbAA and m-HbAS RBCs in recipient mouse tissues (Magnification 20 ×, Green: phalloidin, Blue: DAPI, Red: cy3 RBCs). (b) Individual points represent sequestered cy3-labeled RBCs normalized to nuclei number per section quantified by fluorescence intensity using NIS Elements. Two sections from each recipient mouse organ were analyzed (n = 10 sections per group). Statistical Analysis by two-tailed Mann Whitney U, *p < 0.05, **p < 0.01, lines indicate the median.