Fig. 7.

Increased sequestration of stored h-HbAS RBCs in the spleen following transfusion when compared with stored h-HbAA RBCs. WT C57BL/6 recipient mice (n = 8 per group) were transfused with conventionally stored h-HbAA or h-HbAS RBCs. Kidney, liver and spleen organs were harvested 2 h post-transfusion for confocal imaging. (a) Confocal images showing sequestered h-HbAA and h-HbAS RBCs in recipient mouse tissues (Magnification 20 ×, Green: phalloidin, Blue: DAPI, Red: Glycophorin A: FITC antibody for human RBCs). (b) Individual points represent sequestered h-RBCs normalized to nuclei number per section quantified by fluorescence intensity using NIS Elements. Two sections from each recipient mouse organ were analyzed (n = 16 sections per group). Statistical Analysis by two-tailed Mann Whitney U, **p < 0.01, lines indicate the median.