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. 2016 Jul 30;11:91–100. doi: 10.1016/j.ebiom.2016.07.034

Fig. 1.

Fig. 1

Adiposity and glucose metabolism of BMP4 knockout mice. (A) Growth curve of BMP4 knockout and wild type control mice on HFD from 8 to 26 weeks. n = 6. (B) Representative pictures of mice and fat pads of BMP4 knockout (left panel) and control mice (right panel). (C, D) Fat index (percentage of fat pad weight to the whole body weight) of inguinal WAT and gonadal WAT in BMP4 knockout and control male (C) and female (D) mice. n = 8. (E) H&E staining of inguinal WAT and gonadal WAT from BMP4 knockout and control mice. Scale bar: 25 μm. (F) Quantification of adipocyte diameter of inguinal WAT, gonadal WAT and BAT from BMP4 knockout and control mice. (Data were collected using Image J software from H&E staining section of 3 individual mice, 5 fields per mouse, 10–15 cells per field in each group). (G) Glucose concentrations during an intraperitoneal glucose tolerance test (I) (n = 8) and quantification of AUG (area under curve) from BMP4 knockout and control mice. n = 6–9. (H) Glucose concentrations during an intraperitoneal insulin tolerance test (n = 7) and quantification of AUG from BMP4 knockout and control mice. n = 6–10. BMP4 +/+: wild type control; BMP4 −/−: BMP4 knockout. Data are collected from mice on HFD at age of 6 months except indicated, and expressed as means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.