Skip to main content
. 2016 Jul 30;11:91–100. doi: 10.1016/j.ebiom.2016.07.034

Fig. 2.

Fig. 2

Enhanced ERα signaling in WAT of BMP4 knockout female mice. (A) Serum 17β-estradiol level showed by Elisa in BMP4 knockout and control mice. n = 4. (B) RT-qPCR analysis of WAT for expression of ERα from female BMP4 knockout and control mice on HFD at age of 6 months. n = 5. (C) Representative Western blotting analysis of ERα expression level in adipose tissue from BMP4 knockout and control female mice on HFD at age of 6 months. Relative grey intensity of the band was quantitated using Image J software. n = 6–7. (D) RT-qPCR analysis of WAT for expression of ERα target genes GPX3, LXRα and LXRβ from 6 months old mice on HFD. n = 6. (E) Daily food intake of control and knockout female mice maintained on a HFD, 8 mice per group, weigh each week for 5 weeks. (F) Whole-body oxygen consumption rate (VO2) of control and knockout female mice of 6 months during a 12-hour dark/12-hour light cycle measured in metabolic cage, n = 8. (G) RT-qPCR analysis of WAT for expression of genes of β oxidation from female BMP4 knockout and control mice on HFD at age of 6 months, n = 8. Data are expressed as means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001. BMP4 +/+: wild type control; BMP4 −/−: BMP4 knockout.